Proceedings of the International scientific and practical conference ―Cambridge Education Forum‖ (June 1-3, 2026) / Publisher website: www.naukainfo.com. - Cambridge, United Kingdom, 2026. - 206 p.

84 contrast, biogenic synthesis automatically envelops the nanoparticle in a complex shell composed of fungal biomolecules, including proteins, lipids, and polysaccharides [10, p. 372]. This biomolecular corona ultimately defines the biological identity and physiological behavior of the nanosystem [8, p. 783]. The natural biocorona performs three critical functions that define the utility of BMNs in nanomedicine: – Spatial stabilization : the biocorona prevents the agglomeration of nanoparticles in suspensions and physiological media through robust electrostatic repulsion and steric hindrance [10, p. 5629; 9, p. 2996]. – Reduction of cytotoxicity : the organic envelope effectively masks the inorganic magnetic core (such as magnetite, Fe 3 O 4 ). This makes the BMN "recognizable" to biological systems, reducing opsonization and delaying phagocytic clearance, and significantly reducing oxidative stress and cytotoxicity in cells compared to bare synthetic particles [11, p. 380]. – Surface functionalization : the abundant free amino and carboxyl groups present on the biocorona's surface allow for the straightforward covalent conjugation of targeted chemotherapeutic drugs, antibodies, or fluorescent markers without the need for complex intermediate chemical manipulations [11, p. 382]. In conclusion, the application of mycelial fungi, particularly Aspergillus niger , for the green synthesis of BMNs represents a highly efficient alternative to traditional manufacturing methods. The natural biocorona formed during extracellular mycosynthesis ensures the colloidal stability of the nanoparticles, provides inherent biocompatibility, and offers significant functionalization potential. These characteristics position fungal BMNs as promising candidates for advancing targeted drug delivery, magnetic hyperthermia, and theranostic platforms. REFERENCES: 1. Gorobets O., Gorobets S., Gorobets Yu. Biogenic magnetic nanoparticles: Biomineralization in prokaryotes and eukaryotes. Dekker Encyclopedia of